1. The graph below depicts the growth of a Gram-positive, spore-forming bacterium Bacillus thuringiensis (B. t.). This species normally lives in the soil as well as the alkaline gut of insects. a) Label on the graph the phases of this growth cycle. Below, describe the important features of each phase. b) The graph shown was obtained by culturing B. t. in media containing only minimal amounts of nutrients and salts. Draw a growth curve on the same graph that would result from growing this organism in media highly enriched with nutrients and salts. Label the curve “Rich Media”. Explain your curve. c) Describe what technique you would use to determine the total number of cells present in the culture during the growth period. Log number of bacteria Log number of bacteria


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