Ecological Interactions EXPERIMENT 1: ECOLOGICAL INTERACTIONS Data Tables Table 2: Volume and Concentration Totals Trophic Cylinder oil Volume of Level 1st 2nd 1000 mL 100 mL Post-Lab Questions What does the water in this experiment represent? What does the oil represent? 1. 2. How did the amount of oil change from one trophic level to the next? 3. How does the change in the amount of oil relate to biomagnification? 4. Why would this change in amount of an accumulating substance potentially be of concern in the food chain of an ecosystem? If you would like to read more about issues surrounding bioaccumulation simply search for case studies that address POP (persistent organic pollutants) and biQmagnification in ecosystems Experimreenet Ecological Interactions Experiment Inventory Labware 10 mL Graduated Cylinder 100 mL Graduated Cylinder Materials 10 mL Vegetable Oil Water Note: You must provide the materials listed in "red. EXPERIMENT 1: ECOLOGICAL INTERACTIONS In this experiment, you will demonstrate how substances accumulate in trophic levels through biomagnification PROCEDURE □1. Measure 9 mL of water into the 10 mL graduated cylinder. The graduated cylinder represents a primary producer. 2. Add 1 mL of oil to the 10 mL graduated cylinder containing the water. Note that the oil is insoluble in the water, and thus will accumulate in the water much like some substances accumulate in an organism's tissues. Record the volume of oil in the 10 mL graduated cylinder in Table 2. Pour the oil and water mixture into the 100 mL graduated cylinder. Repeat 1,2 and 4 four more times. Wait until the water and oil has settled, then record the volume of oil in your 100 mL con- tainer in Table 2. □ 3. 4. 5. Ecological Interactions EXPERIMENT 1: ECOLOGICAL INTERACTIONS Data Tables Table 2: Volume and Concentration Totals Trophic Cylinder oil Volume of Level 1st 2nd 1000 mL 100 mL Post-Lab Questions What does the water in this experiment represent? What does the oil represent? 1. 2. How did the amount of oil change from one trophic level to the next? 3. How does the change in the amount of oil relate to biomagnification? 4. Why would this change in amount of an accumulating substance potentially be of concern in the food chain of an ecosystem? If you would like to read more about issues surrounding bioaccumulation simply search for case studies that address POP (persistent organic pollutants) and biQmagnification in ecosystems Experimreenet Ecological Interactions Experiment Inventory Labware 10 mL Graduated Cylinder 100 mL Graduated Cylinder Materials 10 mL Vegetable Oil Water Note: You must provide the materials listed in "red. EXPERIMENT 1: ECOLOGICAL INTERACTIONS In this experiment, you will demonstrate how substances accumulate in trophic levels through biomagnification PROCEDURE □1. Measure 9 mL of water into the 10 mL graduated cylinder. The graduated cylinder represents a primary producer. 2. Add 1 mL of oil to the 10 mL graduated cylinder containing the water. Note that the oil is insoluble in the water, and thus will accumulate in the water much like some substances accumulate in an organism's tissues. Record the volume of oil in the 10 mL graduated cylinder in Table 2. Pour the oil and water mixture into the 100 mL graduated cylinder. Repeat 1,2 and 4 four more times. Wait until the water and oil has settled, then record the volume of oil in your 100 mL con- tainer in Table 2. □ 3. 4. 5.