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CALCULATION: obtain a final Amp concentration of 100 μg/ml in 200ml of pre-warmed LB using a 100 mg/ml stock of ampicillin. CALCULATION: obtain a final Amp concentration of 100 μg/ml in 200ml of pre-warmed LB using a 100 mg/ml stock of ampicillin.
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Initial stock concentration of Ampicillin, C1 = 100 mg/ml = 100 x 103 ug/ml = 105 ug/ml Initial volume, V1 = V ml Final concentration, C2 = 100 ug/ml Final volume, V2 = 200 ml C1V1 = C2V2 105 ug/ml x V = 100 ug/ml x 200 ml V = 0. 2 ml Volume of initial stock solution = 0. 2 ml 0. 2 ml of 100 mg/ml is taken and diluted to 200 ml using pre warmed LB agar to obtain final ampicillin concentration of 100 ug/ml
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