3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO Ara 01-2029 Applied Microbiology and Organic Chemistry 2019 a. Determine the number of viable cells in the culture at 8 h? b. Which dilution tube did you use to perform this analysis and why? c. Calculate the number of generations between 8 h and 14 h of growth (you can assume the culture is in log phase growth). d. Calculate the generation time (in h or min) for this culture. e. Calculate the growth rate (1/h). f. What has happened as far as culture dynamics between 14 h and 20h? 3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO Ara 01-2029 Applied Microbiology and Organic Chemistry 2019 a. Determine the number of viable cells in the culture at 8 h? b. Which dilution tube did you use to perform this analysis and why? c. Calculate the number of generations between 8 h and 14 h of growth (you can assume the culture is in log phase growth). d. Calculate the generation time (in h or min) for this culture. e. Calculate the growth rate (1/h). f. What has happened as far as culture dynamics between 14 h and 20h?