I don't know how to distinguish between the coding/noncoding strand on the problem. I have to put fragment into cut segment and then determine what happened when that occurred and why the experimenter is receiving the observations she's getting. Please do ligation, transcription and translation. (IV). A cloning vector is cut with the restriction endonuclease Sma 1, whose restriction site is C C C (cut in between here, as shown in image) G G G and treated with alkaline phosphatase to prevent the relegation of the plasmid. The synthetic, double-stranded DNA fragment (foreign DNA) with sequence 5' -G G A C T T A C T A C C C A A G T A- OH 3' 3' OH-C C T G A A T G A T G G G T T C A T- 5' is inserted into the Smal site of the vector, whose location with respect to the beta-galactosidase gene in the cloning vector is shown below. (it is a blunt-end ligation!) +H3N-Met - Thr - Met - Ile - Thr - Asn - Ser - Arg - Gly ---> (functional beta-galactosidase gene Starts here.) - ATG | ACC | ATG | ATT | ACG | AAT | TCC | C(arrow points here to cut here) GG | GGA - ↑ Cut here Upon transformation of the ligation mixture into E. coli and in the presence of the indicator X-gal, 52% of the plaques on the plate are blue and 48% are clear. Give the most likely explanation for this result. (Blue colonies are not due to relegation of the plasmid!!) SHOW ALL YOUR WORK! (Include ligation, transcription and translation of the ligated products). HINT: Make sure to identify the coding/non-coding strands!!! You should refer to lab 7 power points for this! You may need more room to work on this! Please attach your answer or write on the back of this page. PLEASE be NEAT!!!! (IV). A cloning vector is cut with the restriction endonuclease Sma 1, whose restriction site is ССС GGG and treated with alkaline phosphatase to prevent the relegation of the plasmid. The synthetic, double-stranded DNA fragment (foreign DNA) with sequence ·G G A C T T A C T A C C C A A G T A-OH 3. OH-CCTGAATGATGGGTTCAT 5' is inserted into the Smal site of the vector, whose location with respect to the β-galactosidase gene in the cloning vector is shown below. it is a blumt-end ligation! H,N-Met-Thr-Met . Ile -Thr . Asn . Ser . Arg -Gly→ finctionalp- galactosidase gene Starts here. Upon transformation of the ligation mixture into E. coli and in the presence of the indicator X-gal, 52% of the plaques on the plate are blue and 48% are clear. Give the most likely explanation for this result. (6 points) SHOW A for this You may need more room to work on this! Please attach your answer or write on the back of this page, PLEASE be NEAT (IV). Acloning vector is cut with the restriction endonuclease Sma, whose restriction site is and treated with alkaline phosphatase to prevent the relegation of the plasmid. The synthetic double-stranded DNA fragment (foreign DNA) with sequence 5 -GGACTTACTACCCAAGT A- OH 3' 3 OH-C CTGAATGATGGGTTCAT- 5" is inserted into the Smal site of the vector, whose location with respect to the β-galactosidase gene in the cloning vector is shown below. i is a blunt-end ligation!) H,N-Met-Thr-Met - Ile -Thr - Asn - Ser -Arg - Gly→ functional β- galactosidase gene Starts here. -ATG ACC ATG ATT ACG AAT TCC cGG GGA- ut her Upon transformation of the ligation mixture into E. coli and in the presence of the indicator X-gal, 52% of the plaques on the plate are blue and 48% are clear. Give the most likely explanation for this result. (6 points) (Blue colonies are not due to relegation of the plasmid!! SHOW ALL YOUR WORK! Include ligation, transcription and translation of the ligated products HINT: Make sure to identify the coding/non-coding strands!!! You should refer to lab 7 power points You may need more room to work on this! Please attach your answer or write on the back of this page . PLEASE be NEAT/!!! Third Position 3-end Coding rad Template and Secand Position DNA UUU Phe UCU Ser UAUTyyUGU C UUC Phe UCC Ser UACT UGC Cs UUA LeuUCA Ser UAA Stop UGA Stop RNA polymerase CUU LeuCCU Pro CAU His CGUArg CUC Leu COC Po CACHs CGC Arg CUA Leu CCA Pro CA GIn CGAArg RNa transcript UU AU AUA Ile MAThr AAA Lys AGA Arg AUG Met ACGThr AGLs AGG Arg GUU VlGCU Ala GAU Asp GGU Gly GUC Val GCC Ala GMAp GGC Gly GUA ValGGAAla GAA GhGGA Gly (IV). A cloning vector is cut with the restriction endonuclease Sma 1, whose restriction site is ССС GGG and treated with alkaline phosphatase to prevent the relegation of the plasmid. The synthetic, double-stranded DNA fragment (foreign DNA) with sequence ·G G A C T T A C T A C C C A A G T A-OH 3. OH-CCTGAATGATGGGTTCAT 5' is inserted into the Smal site of the vector, whose location with respect to the β-galactosidase gene in the cloning vector is shown below. it is a blumt-end ligation! H,N-Met-Thr-Met . Ile -Thr . Asn . Ser . Arg -Gly→ finctionalp- galactosidase gene Starts here. Upon transformation of the ligation mixture into E. coli and in the presence of the indicator X-gal, 52% of the plaques on the plate are blue and 48% are clear. Give the most likely explanation for this result. (6 points) SHOW A for this You may need more room to work on this! Please attach your answer or write on the back of this page, PLEASE be NEAT (IV). Acloning vector is cut with the restriction endonuclease Sma, whose restriction site is and treated with alkaline phosphatase to prevent the relegation of the plasmid. The synthetic double-stranded DNA fragment (foreign DNA) with sequence 5 -GGACTTACTACCCAAGT A- OH 3' 3 OH-C CTGAATGATGGGTTCAT- 5" is inserted into the Smal site of the vector, whose location with respect to the β-galactosidase gene in the cloning vector is shown below. i is a blunt-end ligation!) H,N-Met-Thr-Met - Ile -Thr - Asn - Ser -Arg - Gly→ functional β- galactosidase gene Starts here. -ATG ACC ATG ATT ACG AAT TCC cGG GGA- ut her Upon transformation of the ligation mixture into E. coli and in the presence of the indicator X-gal, 52% of the plaques on the plate are blue and 48% are clear. Give the most likely explanation for this result. (6 points) (Blue colonies are not due to relegation of the plasmid!! SHOW ALL YOUR WORK! Include ligation, transcription and translation of the ligated products HINT: Make sure to identify the coding/non-coding strands!!! You should refer to lab 7 power points You may need more room to work on this! Please attach your answer or write on the back of this page . PLEASE be NEAT/!!! Third Position 3-end Coding rad Template and Secand Position DNA UUU Phe UCU Ser UAUTyyUGU C UUC Phe UCC Ser UACT UGC Cs UUA LeuUCA Ser UAA Stop UGA Stop RNA polymerase CUU LeuCCU Pro CAU His CGUArg CUC Leu COC Po CACHs CGC Arg CUA Leu CCA Pro CA GIn CGAArg RNa transcript UU AU AUA Ile MAThr AAA Lys AGA Arg AUG Met ACGThr AGLs AGG Arg GUU VlGCU Ala GAU Asp GGU Gly GUC Val GCC Ala GMAp GGC Gly GUA ValGGAAla GAA GhGGA Gly