Expert Q&A Done 1. Is this strand the sense strand or anti-sense strand? What is your evidence (How did you know)? So is this the template or coding strand? 2. How would you locate the general area of the promoter? Hint: what sequence would you look for? Underline the sequence and label with an (a). What sequence would you look for if the complementary strand was provided? (Always write answers in 5 to 3" unless otherwise 3. How many exons are there? introns? How can you tell? Draw an arrow to one 5'ss and one 3'ss, label with (b) and (c), respectively. 4 How would you locate the ORF? (Hint: What sequence(s) would you look for Draw a bracket around the ORF and label with a (d). 5. Draw a bracket around the 5UTR and label it (b). What is the significance of this region, ie. what role does it play in gene expression? 6. Draw a bracket around the 3'UTR and label it (b). What is the significance of this region, ie. what role does it play in gene expression? 7. How would you locate the general area of the terminator? Hint: what sequence(s) would you look for? Underline the sequence and label with an (e). What if the complementary strand was provided, what sequence would you look for? (Always write answers in 5' to 3' unless otherwise the sequence of the mature RNA strand. 8. Write 9. Write the primary sequence of the polypeptide (abel N-terminus and C-terminus)) USING 3-LETTER and 1-LETTER Amino Acid CODE 10. What effect would a Gà T substitution (underlined/bold G) have on the protein product? Name the type of mutation ( any) and characterize it as conservative or non-conservative? Explain your reason for both answers. BIOL 103-S19 (DO12) 11. What effect would a Gà C substitution product? Name the type of mutation (f any) and characterize it as conservative or non- conservative? Explain your reason for both 12. What effect would a Tà G substitution highlighted/bold T) have on the protein production? Name the type of mutation ( any) and characterize it as conservative or non-conservative? Explain your reason for both answers. 13. What effect would a Cà T substitution (underlined/bold C)have on the protein production? Name the type of mutation (f any) and characterize it as conservative or non-conservative? Explain your reason for both G) have on the protein 5 'gegaatgatataaactggetggcttagCGTGCAATGGGCAGAATT ACGtcgaggetaGCAAATGACGtaatgaGAAGGGCTTGCGAATAACT Expert Q&A Done 1. Is this strand the sense strand or anti-sense strand? What is your evidence (How did you know)? So is this the template or coding strand? 2. How would you locate the general area of the promoter? Hint: what sequence would you look for? Underline the sequence and label with an (a). What sequence would you look for if the complementary strand was provided? (Always write answers in 5 to 3" unless otherwise 3. How many exons are there? introns? How can you tell? Draw an arrow to one 5'ss and one 3'ss, label with (b) and (c), respectively. 4 How would you locate the ORF? (Hint: What sequence(s) would you look for Draw a bracket around the ORF and label with a (d). 5. Draw a bracket around the 5UTR and label it (b). What is the significance of this region, ie. what role does it play in gene expression? 6. Draw a bracket around the 3'UTR and label it (b). What is the significance of this region, ie. what role does it play in gene expression? 7. How would you locate the general area of the terminator? Hint: what sequence(s) would you look for? Underline the sequence and label with an (e). What if the complementary strand was provided, what sequence would you look for? (Always write answers in 5' to 3' unless otherwise the sequence of the mature RNA strand. 8. Write 9. Write the primary sequence of the polypeptide (abel N-terminus and C-terminus)) USING 3-LETTER and 1-LETTER Amino Acid CODE 10. What effect would a Gà T substitution (underlined/bold G) have on the protein product? Name the type of mutation ( any) and characterize it as conservative or non-conservative? Explain your reason for both answers. BIOL 103-S19 (DO12) 11. What effect would a Gà C substitution product? Name the type of mutation (f any) and characterize it as conservative or non- conservative? Explain your reason for both 12. What effect would a Tà G substitution highlighted/bold T) have on the protein production? Name the type of mutation ( any) and characterize it as conservative or non-conservative? Explain your reason for both answers. 13. What effect would a Cà T substitution (underlined/bold C)have on the protein production? Name the type of mutation (f any) and characterize it as conservative or non-conservative? Explain your reason for both G) have on the protein 5 'gegaatgatataaactggetggcttagCGTGCAATGGGCAGAATT ACGtcgaggetaGCAAATGACGtaatgaGAAGGGCTTGCGAATAACT