#1-6 1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count given that you obtained 20 colonies using 100uL of a sample with D.F of 10 г. (О.С.D-: CF.U(Volume plated) X (Dilution)) (1 pts) a. 20K cells/mL b. 2k cells/mL c. 20 cells/mL 4. Calculate the D.F if 10μL of a sample with D. F of 10-2 was added to 990μL of water. (1 pt) a. 10-3 b. 10-6 c. 104 5. What is the primary advantage of viable count over a direct count? (1 pt) Using a direct count, you've counted 45 cells within a cellometer containing 44 squares and a volume of 3 x 106 cm3. The solution you've prepared is 200 ul of bacteria, 300 ul of stain, and 500 μ1 of water. What is the original cell density? (Hint: Calculate the Dilution factor first, then just plug in the values to determine OCD for a direct count. (3 pts) 6. 1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count given that you obtained 20 colonies using 100uL of a sample with D.F of 10 г. (О.С.D-: CF.U(Volume plated) X (Dilution)) (1 pts) a. 20K cells/mL b. 2k cells/mL c. 20 cells/mL 4. Calculate the D.F if 10μL of a sample with D. F of 10-2 was added to 990μL of water. (1 pt) a. 10-3 b. 10-6 c. 104 5. What is the primary advantage of viable count over a direct count? (1 pt) Using a direct count, you've counted 45 cells within a cellometer containing 44 squares and a volume of 3 x 106 cm3. The solution you've prepared is 200 ul of bacteria, 300 ul of stain, and 500 μ1 of water. What is the original cell density? (Hint: Calculate the Dilution factor first, then just plug in the values to determine OCD for a direct count. (3 pts) 6.