You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested in lowly expressed in your cells, therefore, you need to make sure each sample has a final pro of 50 ug. With the information provided, complete the chart below. (16 points) awesome Cell Biology lab that you should never load the maximum i into each well tein concentration Protein concentration is protein .Lof 3540 ug/mL HL protein TL of water -μL 4x buffer 31.5 4315 4.375 4315 3150 ug/mL 2975 ㎍/mL 31.5 31s Before the prepared samples from question 7 are loaded onto the SDS-PAGE gel, we must boil the sample for 5 minutes. Why are the samples boiled? And why does this boiling take place in the sample buffer? (6 points) 8 You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested in lowly expressed in your cells, therefore, you need to make sure each sample has a final pro of 50 ug. With the information provided, complete the chart below. (16 points) awesome Cell Biology lab that you should never load the maximum i into each well tein concentration Protein concentration is protein .Lof 3540 ug/mL HL protein TL of water -μL 4x buffer 31.5 4315 4.375 4315 3150 ug/mL 2975 ㎍/mL 31.5 31s Before the prepared samples from question 7 are loaded onto the SDS-PAGE gel, we must boil the sample for 5 minutes. Why are the samples boiled? And why does this boiling take place in the sample buffer? (6 points) 8