3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the other regions of the DNA
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