Exercise 1. Assuming no further physiological changes occur, calculate the daily (24 hour) carbon balance for leaves of the HL- and LL-grown plants under the following experimental conditions: 1A. Bright light treatment. Plants are kept at a light-level of 200 umol/m2/s for 2 hours, then switched to a light-level of 150°umol/ma/s for 10 hours, then switched back to a light-level of 200 μmol/㎡/s for 2 hours, and finally, left in darkness (0 μmol/m2/s) for 10 hours. This light regime simulates the light conditions in a sunny habitat like a tropical savanna or a semi-arid shrubland. 1B. Dim light treatment. Plants are kept at a light-level of so umol/m2/s for 2 hours, then switched to a light level of 200 μ mol/m2/s for 10 hours, then switched back to a light level of 50 um 1/m2/s for 2 hours, and finally, left in darkness (0 umol/m2/s) for 10 hours. This light regime simulates the light conditions on the floor of a tropical or temperate forest (in the summer). Results for exercise 1 (effect of biochemical acclimation): Daily CO2 balance of HL grown leaves in bright light treatment;mol COlm2 Daily CO2 balance of LL grown leaves in bright light treatmentmol co/m2 Daily CO2 balance of HL grown leaves in dim light treatmentmol CO/m mol CO2/m2 -- -- Daily CO2 balance of LL grown leaves in dim light treatment; Modeling the effects of blochemical and anatomical acclimation to high-light vs low-light conditions on leaf and plant daily carbon balance. (Modifiled from "Analyzing data S.1" in Bowman et al 4E, pg 116). Many plants modify their leaf biochemistry and leaf anatomy depending upon the light conditions they grow under. These acclimation responses presumably optimize the leaves for improved carbon gain under the respective light environments. The figure below from Chapter 5 of your textbook (after Bjorkman 1981) shows the net photosynthetic rates across a range of light conditions for the same species of plant experimentally grown under high-light (HL grown; 920 umol/m2/s photosynthetically active radiation) and low-light (LL grown; 92 μmol/m2/s). In the following exercise you will use these curves to model the effects of acclimation on daily (24 hour) carbon balance for both HL-and LL-grown plants. You will calculate the 24h carbon balance for both kinds of plants placed under contrasting experimental light treatment designed to simulate a 'real world' bright-light habitat and low-light habitat. balance 40 30 Grown in high-light conditions Grown in low-light conditions 500 1,000 1,500 2,000 2,500 Photosynthetically active irradiance (umol/m2/s) Exercise 1. Assuming no further physiological changes occur, calculate the daily (24 hour) carbon balance for leaves of the HL- and LL-grown plants under the following experimental conditions: 1A. Bright light treatment. Plants are kept at a light-level of 200 umol/m2/s for 2 hours, then switched to a light-level of 150°umol/ma/s for 10 hours, then switched back to a light-level of 200 μmol/㎡/s for 2 hours, and finally, left in darkness (0 μmol/m2/s) for 10 hours. This light regime simulates the light conditions in a sunny habitat like a tropical savanna or a semi-arid shrubland. 1B. Dim light treatment. Plants are kept at a light-level of so umol/m2/s for 2 hours, then switched to a light level of 200 μ mol/m2/s for 10 hours, then switched back to a light level of 50 um 1/m2/s for 2 hours, and finally, left in darkness (0 umol/m2/s) for 10 hours. This light regime simulates the light conditions on the floor of a tropical or temperate forest (in the summer). Results for exercise 1 (effect of biochemical acclimation): Daily CO2 balance of HL grown leaves in bright light treatment;mol COlm2 Daily CO2 balance of LL grown leaves in bright light treatmentmol co/m2 Daily CO2 balance of HL grown leaves in dim light treatmentmol CO/m mol CO2/m2 -- -- Daily CO2 balance of LL grown leaves in dim light treatment; Modeling the effects of blochemical and anatomical acclimation to high-light vs low-light conditions on leaf and plant daily carbon balance. (Modifiled from "Analyzing data S.1" in Bowman et al 4E, pg 116). Many plants modify their leaf biochemistry and leaf anatomy depending upon the light conditions they grow under. These acclimation responses presumably optimize the leaves for improved carbon gain under the respective light environments. The figure below from Chapter 5 of your textbook (after Bjorkman 1981) shows the net photosynthetic rates across a range of light conditions for the same species of plant experimentally grown under high-light (HL grown; 920 umol/m2/s photosynthetically active radiation) and low-light (LL grown; 92 μmol/m2/s). In the following exercise you will use these curves to model the effects of acclimation on daily (24 hour) carbon balance for both HL-and LL-grown plants. You will calculate the 24h carbon balance for both kinds of plants placed under contrasting experimental light treatment designed to simulate a 'real world' bright-light habitat and low-light habitat. balance 40 30 Grown in high-light conditions Grown in low-light conditions 500 1,000 1,500 2,000 2,500 Photosynthetically active irradiance (umol/m2/s)


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