The reaction catalysed by pyruvate kinase is: K'eg 3.63 x 105 Phosphoenolpyruvate + ADP -> pyruvate ATP a) Calculate the AG°" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: ATP+H20ADP P AG-30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate -> pyruvate Pi Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 μΜ, 1.85 mM and 140 μM respectively. Given that the ΔG for the pyruvate kinase reaction is -23.0 kJ/mol under these conditions, what is the steady-state concentration of pyruvate? Show your working. 3 marks d) Based on your answer to (c) do you think the pyruvate kinase reaction reaches equilibrium in a cell? Explain your answer. 2 marks The reaction catalysed by pyruvate kinase is: K'eg 3.63 x 105 Phosphoenolpyruvate + ADP -> pyruvate ATP a) Calculate the AG°" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: ATP+H20ADP P AG-30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate -> pyruvate Pi Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 μΜ, 1.85 mM and 140 μM respectively. Given that the ΔG for the pyruvate kinase reaction is -23.0 kJ/mol under these conditions, what is the steady-state concentration of pyruvate? Show your working. 3 marks d) Based on your answer to (c) do you think the pyruvate kinase reaction reaches equilibrium in a cell? Explain your answer. 2 marks