Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA added? (3 marks) If the highest efficiency obtainable by an optimal method is around 1 x 108 transformants per μg and a poor efficiency is around 1 x 10, how does your transformation efficiency compare? (1 mark) 2. You need 200 ml of 0.7% agarose to pour your gel. You are provided with agarose powder, a 5 x TBE solution, a 20,000X GelRed Nucleic Acid stain, and water. How much of each of these four components is required in your mixture to make the agarose gel? (8 marks) If you would like to run 20 ul of your PCR product in the above gel, how much 6X Gel Loading dye you need to prepare your sample? (2 marks) (Total 10 marks) Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA added? (3 marks) If the highest efficiency obtainable by an optimal method is around 1 x 108 transformants per μg and a poor efficiency is around 1 x 10, how does your transformation efficiency compare? (1 mark) 2. You need 200 ml of 0.7% agarose to pour your gel. You are provided with agarose powder, a 5 x TBE solution, a 20,000X GelRed Nucleic Acid stain, and water. How much of each of these four components is required in your mixture to make the agarose gel? (8 marks) If you would like to run 20 ul of your PCR product in the above gel, how much 6X Gel Loading dye you need to prepare your sample? (2 marks) (Total 10 marks)