If you could briefly answer these questions so I can understand. I seem to be stuck on 8-10. But if you could still provide an answer for 6-7 so i can check my answers. Thankyou. tl d at b peeip ri Ht nas nech ntehpv cowa rdele td.no 1I a. gc peoP sh e.. 1tr n st rs le go a l G r 1 as edi 6pP pi si S) nhl es i d 0 ei ly d ce sa y ene nynd e0h cel s Ittd , ee 7654321 oeienbhce ls suun) Bu-pula dd i nup rd 11 b sui rintnio 5 nvec Q6. How is the binding of HPRG to heparin dependent on pH? Q7. pK of histidine in HPRG is approximately the pH when half of the HPRG is bound to heparın. From the graph, this pK is: Q8. The side chain pK of free histidine residues is 6.0. So at pH 6.0, is the histidine side Q9. What happens to the pH dependence of HPRG binding to heparin, when DEPC is Q10. The side chain pK of free histidine residues is 6.0. So at pH 6.0, is the histidine side chain in HPRG charged or neutral? added? chain in HPRG charged or neutral when DEPC is present? tl d at b peeip ri Ht nas nech ntehpv cowa rdele td.no 1I a. gc peoP sh e.. 1tr n st rs le go a l G r 1 as edi 6pP pi si S) nhl es i d 0 ei ly d ce sa y ene nynd e0h cel s Ittd , ee 7654321 oeienbhce ls suun) Bu-pula dd i nup rd 11 b sui rintnio 5 nvec Q6. How is the binding of HPRG to heparin dependent on pH? Q7. pK of histidine in HPRG is approximately the pH when half of the HPRG is bound to heparın. From the graph, this pK is: Q8. The side chain pK of free histidine residues is 6.0. So at pH 6.0, is the histidine side Q9. What happens to the pH dependence of HPRG binding to heparin, when DEPC is Q10. The side chain pK of free histidine residues is 6.0. So at pH 6.0, is the histidine side chain in HPRG charged or neutral? added? chain in HPRG charged or neutral when DEPC is present?
Q 6 ans: A plot of the amount of HPRG bound at equilibrium versus pH resembles an acid-base titration curve with human HPRG having a pK a of 6. 8, near that of histidine. An increase in signal due to the specific binding of IgG to HPRG already bound to heparin is observed at pH 6. 7 and to a limited extent at, but not above, pH 7. 0, in good agreement with the results obtained with the purified system. Q9 ans: HPRG treated with excess diethylpyrocarbonate (DEPC) to chemically modify histidine residues does not bind, supporting the importance of histidine in the interaction with heparin.
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