Please do everything like you were answering a test. Don’t attempt if youre not going to do all parts. Do it ASAP and I will give you a good rating. If not I will report you. Thank you so much for being the best. Show work if necessary and be concise. There’s no way for me to separate so do all parts. do all parts please. I cant seperate it because it will be refunded. 2. i) (10 points) The enzyme "A" catalyzes the hydrolysis of the peptide A-G-W in 10mM phosphate buffer at pH 4. Assuming this enzyme follows Michaelis-Menten kinetics, calculate the Vmax of the enzyme if KM 3mM, Vo 150mMol/min and [S]-0.5mM. i) (5 points) What is the initial velocity of the enzyme catalyzed reaction as a percentage of Vmax, if [S] 1.5 KM points) An inhibitor "T" was added to the reaction mixture. The Vmas measured was 800mMol/min olmin and the KM-2.5 mM. What type of an inhibitor was it? V)(extra credit) For the same reaction in question iii, if the y intercept of the graph in the for that presence of 1mM of inhibitor was given by (+VI) Vmax,, calculate K inhibitor. V) (5 points) Sketch the Lineweaver-Burk plot in the presence and in the absence of an inhibitor? Label axes, curves, important intercepts, slopes. 2. i) (10 points) The enzyme "A" catalyzes the hydrolysis of the peptide A-G-W in 10mM phosphate buffer at pH 4. Assuming this enzyme follows Michaelis-Menten kinetics, calculate the Vmax of the enzyme if KM 3mM, Vo 150mMol/min and [S]-0.5mM. i) (5 points) What is the initial velocity of the enzyme catalyzed reaction as a percentage of Vmax, if [S] 1.5 KM points) An inhibitor "T" was added to the reaction mixture. The Vmas measured was 800mMol/min olmin and the KM-2.5 mM. What type of an inhibitor was it? V)(extra credit) For the same reaction in question iii, if the y intercept of the graph in the for that presence of 1mM of inhibitor was given by (+VI) Vmax,, calculate K inhibitor. V) (5 points) Sketch the Lineweaver-Burk plot in the presence and in the absence of an inhibitor? Label axes, curves, important intercepts, slopes.