In this lab, you prepared a buffer and then determined its pH, but in a laboratory setting it is usually the other way around – you need a buffer at a particular pH, and this dictates its preparation. Describe how you would prepare 500. mL of 0.1500M HEPES at pH = 7.80, given appropriate glassware, HEPES (mw = 238.3g/mol, pKa = 7.48) and HEPES sodium salt (mw = 260.3 g/mol) at 25°C. Your answer should include all relevant calculations. (Note: The moles of each component must add to the total buffer moles. As an example, for 1L of a 1M buffer, A-+ HA = 1 mol)